These results show that exogenous overexpression of miR- 645 promotes the invasion and migration of HNSCC cells in vitro. Furthermore, we have investigated the mechanism of miR- 645 in HNSCC cancer cell lines.
We found that miR- 645 levels were up-regulated in HNSCC tissues and highly invasive cell lines. In this study, we examined the expression of miR- 645 in HNSCC samples by gene chips and further confirmed in HNSCC samples and HNSCC cell lines using real-time PCR. miR- 645 has been reported to be disrupt-expression in pathological states, however, the role of miR- 645 in metastasis of cancer has not been reported. There are some biomarkers for the classification diagnosis, individual treatment and prognosis of HNSCC investigated by miRNAs array and studies have shown altered miRNAs profiles in HNSCC compared to their normal tissue. The presence of lymph node metastasis affects more than 50 % of HNSCC patients and it is one of the most important prognostic indicators associated with poor long survival rates. Survival rates for HNSCC have remained unchanged throughout the last three decades, and half of all cases die within 5 years of diagnosis. Head and neck squamous cell carcinoma (HNSCC) ranks sixth among cancers worldwide, includes tumors of the oral cavity, oropharynx, and larynx. Although the number of verified human miRNAs is still expanding, the functions of only a few of them have been described. Several miRNAs are up-regulated in specific tumors appears a general trait of human cancers which playing a causal role in the transformed phenotype, and the mechanisms are remain to be known further. Many evidences suggest that the regulatory capacity of miRNAs is dysregulated and exploited in malignant tumors. Subsets of miRNAs have been identified as potential diagnostic and prognostic markers in malignant tumors. Nucleotides 2–7, from the 5′ end of the miRNAs, are referred to as the “seed” and are critical for hybridization to the targets, It has been firmly established that miRNAs regulate many key cellular processes such as cell growth, differentiation and apoptosis. MicroRNAs (miRNAs) are endogenous RNAs that play important gene-regulatory roles in animals via sequence-specific interactions with the 3′UTR of cognate mRNA targets, causing suppression of translation and mRNA decay.
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